Real-time Analysis of Photoassimilate Translocation in Intact Eggplant Fruit using ^<11>CO_2 and a Positron-emitting Tracer Imaging System
スポンサーリンク
概要
- 論文の詳細を見る
A recently developed positron-emitting tracer imaging system (PETIS) noninvasively produces quantitative realtime images of the movement of various compounds in plants. To clarify the mechanism of the growth and development of the fruit of eggplant (Solarium melongena L.), we fed ^<11>CO_2 to a leaf and monitored the translocation of ^<11>C-labeled photoassimilate into the fruit by PETIS. Continuous images of the translocation of [^<11>C]photoassimilate from the leaf to the fruit and inside the fruit were produced, with a shorter time resolution than reported previously. ^<11>C signal intensity in the fruit increased gradually, and its distribution was not uniform. The fruits were sliced after PETIS measurement and exposed to the imaging plates of a bio-imaging analyzer system. The resultant images showed the localization of ^<11>C activity inside the fruit, and indicated that translocation of photoassimilate within the fruit was not uniform. The velocity of photoassimilate translocation in the peduncle and changes in the rate of translocation of photoassimilate in the fruit were estimated by analysis of PETIS data. The velocity of photoassimilate translocation through the peduncle was estimated as 1.17 cm・min^<-1>. About 60 min after the start of ^<11>CO_2 feeding, the ^<11>C activity of the fruit began to increase, and by 120 min it had reached about 8% of feeding ^<11>CO_2. These results indicate that it took about 60 min for the first [^<11>C]photoassimilates to reach the fruit. Real time observation of photoassimilate translocation within a fruit has never been reported. PETIS may be a powerful tool for revealing the mechanisms of fruit development and maturity.
- 園芸学会の論文
著者
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Suzui Nobuo
Plant Positron Imaging Group Quantum Beam Science Directorate Japan Atomic Energy Agency
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Fujimaki Shu
Plant Positron Imaging Group Quantum Beam Science Directorate Japan Atomic Energy Agency
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Ishii Satomi
Plant Positron Imaging Group Quantum Beam Science Directorate Japan Atomic Energy Agency:graduate Sc
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Kawachi Naoki
Plant Positron Imaging Group Quantum Beam Science Directorate Japan Atomic Energy Agency
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Matsuhashi Shinpei
Japan Atomic Energy Agency
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Kikuchi Kaori
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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Ishii Satomi
Japan Atomic Energy Agency
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Fujimaki Shu
Japan Atomic Energy Agency
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Suzui Nobuo
Japan Atomic Energy Agency
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Honda Ichiro
National Institute of Vegetable and Tea Science, National Agriculture and Food Research Organization
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Shishido Yoshihiro
MKV Platech Co. Ltd.
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Kawachi Naoki
Japan Atomic Energy Agency
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Kikuchi Kaori
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Honda Ichiro
National Institute Of Crop Science (nics)
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Honda Ichiro
National Institute Of Vegetable And Tea Science National Agriculture And Food Research Organization
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Honda Ichiro
National Agriculture Research Center
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