ヘッドスペース・ガスクロマトグラフ(HS・GC)法による尿中アセトン,メタノール,およびメチルエチルケトンの定量

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Using HS・GC, We have succeeded in simultaneous determination of Ac, MeOH and MEK in urine without any complicated pretreatment or correction by internal standard. Moreover, in order to lower the detection limits of these materials, study was made on the salting out effect using 14 kinds of salts. As pretreatment, 2.0ml of urine, 3.0g of sodium sulfate and small sized magnetic stirrer are put into vial, which is sealed by septum. This is then heated for 10min in warm bath of 50℃. In order to dissolve the added salts as much as possible, the specimen is stirred by the stirrer. After cooling the liquid to room temperature, the specimen is analysed by HS・GC. The results showed that sodium sulfate was excellent synthetically. 1) Using the urine of workers not exposed to organic solvents three kinds of urine having specific gravity of 1.010, 1.024 and 1.034 were prepared and mixed standard organic solvents (Ac, MeOH and MEK) were added. Recovery percentages and coefficients of variation were calculated. The results showed that recovery percentages ranged from 92.0 to 101.7% and coefficients of variation from 0.2 to 4.6%. 2) The regression equations of standard curves were satisfactory with y=9053x-200 (r=0.999, n=12) for Ac, y=801x-400 (r=0.999, n=12) for MeOH, and y=15488x-277 (r=0.999, n=12) for MEK. 3) The detection limits calculated by IUPAC formula were 0.0092mg/l for Ac, 0.11mg/l for MeOH and 0.0063mg/l for MEK. These results indicated that this method is superior to other methods because the pretreament is very simple, specificity is excellent, analysis by standard curves is possible, and this method is not affected by specific gravity of the urine.
社団法人日本産業衛生学会の論文
1992-05-20

ヘッドスペース・ガスクロマトグラフ(HS・GC)法による尿中アセトン,メタノール,およびメチルエチルケトンの定量

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