Optimization of Critical Medium Components Using Response Surface Methodology for Phenazine-1-Carboxylic Acid Production by Pseudomonas sp. M-18Q(BIOCHEMICAL ENGINEERING)

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概要

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• The optimal flask-shaking batch fermentation medium for phenazine-1-carboxylic acid(PCA) production by Pseudomonas sp. M-18Q, a qscR chromosomal inactivated mutant of the strain M18 was studied using statistical experimental design and analysis. The Plackett-Burman design(PBD) was used to evaluate the effects of eight medium components on the production of PCA, which showed that glucose and soytone were the most significant ingredients(P<0.05). The steepest ascent experiment was adopted to determine the optimal region of the medium composition. The optimum composition of the fermentation medium for maximum PCA yield, as determined on the basis of a five-level two-factor central composite design(CCD), was obtained by response surface methodology(RSM). The high correlation between the predicted and observed values indicated the validity of the model. A maximum PCA yield of 1240 mg/l was obtained at 17.81 g/l glucose and 11.47 g/l soytone, and the production was increased by 65.3% compared with that using the original medium, which was at 750 mg/l.

• 2008-03-25

著者

• Zhang Xue-hong

  Key Laboratory Of Microbial Metabolism Shanghai Jiao Tong University

• Xu Yu-quan

  Key Laboratory Of Microbial Metabolism Shanghai Jiao Tong University

• Yuan Li-Li

  Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University

• Li Ya-Qian

  Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University

• Wang Yi

  Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University

• Li Ya-qian

  Key Laboratory Of Microbial Metabolism Shanghai Jiao Tong University

• Yuan Li-li

  Key Laboratory Of Microbial Metabolism Shanghai Jiao Tong University

• Wang Yi

  Key Laboratory Of Microbial Metabolism Shanghai Jiao Tong University

• Wang Yi

  Key Laboratory Of Marine Drugs Chinese Ministry Of Education Institute Of Marine Drugs And Food Ocean University Of China

関連論文

• Optimization of Critical Medium Components Using Response Surface Methodology for Phenazine-1-Carboxylic Acid Production by Pseudomonas sp. M-18Q(BIOCHEMICAL ENGINEERING)
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