P^<32>標識赤血球による循環血液(血球)量測定法に關する基礎的研究
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The comparative studies on the method of the determination of circulating blood volume have been carried out with dye T-1824 and labeled red cells (CO, Fe^<59> and P^<32>) by various investigators. The relationships between the radioactivity disappearance curve and the conditions of making up labeled red cell, however, have not been thoroughly explained. The author intended to make clear these relationships ; and 38 determinations of blood volume were carried out by following precisely the radioactivity disappearance curve in combination with T-1824. The results were as follows. 1. Some marked differences in the type of radioactivity disappearance curve were recognized by the conditions of preparing labeled red cell. Only when the curve was expressed as an exponential function, a definite distribution of P^<32> in the labeled red cell was ascertained, and the determination of radioactivity at time of injection was possible by the method of extrapolation of this function. 2. The ratio of red cell volume with P^<32> method to that with T-1824 was 0.87±0.03. 3. The ratio of body hematocrit to average venous hematocrit was 0.93±0.02. 4. The direct method in the determination of circulating blood volume with P^<32> method is sufficiently useful in the praxis and enough accurate and eliminates the error of centrifugation. 5. The author discussed the time of withdrawal of blood specimens if limited once, and also presented the values of circulating red cell and blood volume on healthy persons and patients with cardiac failure.
- 社団法人日本循環器学会の論文
- 1952-11-20
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