P229 電気化学的な培養法を用いたグリセロール微生物変換に関する研究(ポスター発表)

概要

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Glycerol is produced as a main by-product under the process of the biodiesel fuel(BDF) production. In the near future, the increase of BDF production will cause surplus of glycerol worldwide. Therefore, there is a demand for effective methods of conversion glycerol to more valuable materials. We have developed a system of the bioconversion of glycerol to ethanol, hydrogen gas and electricity using Enterobacter aerogenes NBRC 12010. In the first of this conference, we reported that electrochemical fermentation, extracting electron from bacterial cells under fermentative condition, activated the bioconversion of glycerol from the process of BDF production in contrast with only fermentation. However, it was not clear the mechanism how electrochemical fermentation activated this bioconversion. In this report, we demonstrated this mechanism as follows. In the cells of E. aerogenes NBRC 12010, glycerol dehydrogenase(GDH) catalyzed the first reaction of the glycerol metabolism, and its activity was regulated by the balance of cellular NAD^+ and NADH. As a result, this balance also controlled the whole glycerol metabolism. Electrochemical reaction could oxidize cellular NADH to NAD^+, so that glycerol metabolism was activated.
一般社団法人日本エネルギー学会の論文
2007-01-16