Effects on Porcine Mesenchymal Stem Cells When Cultured in Autologous Versus Fetal Bovine Serum

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概要

• 論文の詳細を見る

• The use of FBS (Fetal Bovine Serum) is accompanied by a risk of disease trans-mission. The aim of this study was to evaluate autologous serum (AS) as an alternative culture medium for tissue engineering. Alkaline phosphatase (ALP) activity, DNA production (cell proliferation), and production of mineralized bone matrix were assessed when porcine mesenchymal stem cells (pMSCs) were cultured in autologous versus fetal bovine serum. Cells were cultured as follows: 1) DMEM (Dulbecco's modified high glucose eagle's medium), osteogenic supplements (OS: 100 nM dexamethasone, 50μg/ml ascorbic acid, and 10mM beta- glycerophosphate), and 10% active autologous serum (AAS); 2) DMEM, OS, and 10% inactivated (heated) autologous serum (IAS); 3) DMEM, OS, and 10% FBS (control). At 3, 7, 14 and 21 days in culture, Alkaline phosphatase (ALP) activity, DNA production (cell proliferation), and production of mineralized bone matrix were measured. ALP activity and DNA production were greater at all time points in the FBS (control) group versus the experimental (AAS and IAS) groups. However, ALP activity relative to DNA production in the AAS and IAS groups was equal to the FBS group.

• 日本再生歯科医学会の論文
• 2007-09-30

著者

• Vacanti Joseph

  Pediatric Surgery Tissue Engineering And Organ Fabrication Laboratory Massachusetts General Hospital

• ABUKAWA Harutsugi

  Department of Oral and Maxillofacial Surgery, Tokyo Medical University Hospital

• BAUMANN Arnulf

  Hospital of Cranio-Maxillofacial and Oral Surgery, Medical University of Vienna

• KABAN Leonard

  Department of Oral and Maxillofacial Surgery. Massachusetts General Hospital and Harvard School of D

• TROULIS Maria

  Minimally Invasive Oral and Maxillofacial Surgery Program, Skeletal Biology Research Center, Departm

• Kaban Leonard

  Department Of Oral And Maxillofacial Surgery. Massachusetts General Hospital And Harvard School Of D

• Baumann Arnulf

  Hospital Of Cranio-maxillofacial And Oral Surgery Medical University Of Vienna

• Troulis Maria

  Minimally Invasive Oral And Maxillofacial Surgery Program Skeletal Biology Research Center Departmen

• Abukawa Harutsugi

  Department Of Oral And Maxillofacial Surgery Tokyo Medical University

• Abukawa Harutsugi

  Department Of Oral And Maxillofacial Surgery Tokyo Medical University Hospital

関連論文

• Effects on Porcine Mesenchymal Stem Cells When Cultured in Autologous Versus Fetal Bovine Serum
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