Fabrication of Electrospun Thai Silk Fibroin Nanofiber and Its Effect on Human Gingival Fibroblast : A Preliminary Study

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概要

• 論文の詳細を見る

• To make the tissue under concept of tissue engineering, it is necessary to cultivate cells in proper scaffolds. Silk fibers are useful materials for range of biomedical applications as well as scaffolds for tissue engineering. The final goal of our project is to use natural Thai silk fibers as a novel scaffold for tissue replacement and/or regeneration. Objectives: The aims of this study are to fabricate electrospun Thai silk fibroin nanofibers and test for their cytotoxic effect on human gingival fibroblast cells. Methods: Protein fibroin from Thai Bombyx mori cocoons was extracted by Na_2CO_3, CaCl_2/C_2H_5OH/H_2O solution and was then dialyzed in deionized water at 4℃ for 72hrs. Subsequently, the dialyzed product was lyophilized to yield a sponge. After dissolved fibroin sponge in formic acid, the fibroin nanofibers were obtained by electrospinning under 15KVp, 17mA at loading rate 0.5ml/hr. The electrospun fibroin nanofibers were studied for their ultrastructures as well as cy-totoxicity by microscopy and cell culture system. Results: Based on this protocol, the fibroin nanofibers demonstrated grayish-white highly order fibers. The results from the co-culture of fibroin nanofibers with human gingival fibroblasts demonstrated that cells could proliferate and attach to the nanofibers suggested non-toxic property of the fibers. In conclusion, we successfully fabricated the Thai silk fibroin nanofibers which were not toxic to human gingival fibroblasts in vitro. After optimum physical property adjustment and extensive examination in vivo, the Thai silk fibroin nanofibers might be able to use as natural, non-toxic and promising scaffolds for tissue engineering.

• 日本再生歯科医学会の論文
• 2007-09-30

著者

• Damrongrungruang Teerasak

  Department Of Oral Diagnosis Faculty Of Dentistry Khon Kaen University

• SIRITAPETAWEE Mookhda

  Department of Oral Diagnosis, Faculty of Dentistry, Khon Kaen University

• KAMANARONG Kimaporn

  Department of Orthopaedic Surgery, Faculty of Medicine, Khon Kaen University

• LIMMONTHON Saowaluck

  Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Khon Kaen University

• RATTANATHONGKOM Areeya

  Department of Oral Biology, Faculty of Dentistry, Khon Kaen University

• MAENSIRI Santi

  Department of Physics, Faculty of Sciences, Khon Kaen University

• NUCHDAMRONG Suporn

  Department of Biochemistry, Faculty of Sciences, Khon Kaen University

• Maensiri Santi

  Department Of Physics Faculty Of Sciences Khon Kaen University

• Nuchdamrong Suporn

  Department Of Biochemistry Faculty Of Sciences Khon Kaen University

• Kamanarong Kimaporn

  Department Of Orthopaedic Surgery Faculty Of Medicine Khon Kaen University

• Limmonthon Saowaluck

  Department Of Oral And Maxillofacial Surgery Faculty Of Dentistry Khon Kaen University

• Siritapetawee Mookhda

  Department Of Oral Diagnosis Faculty Of Dentistry Khon Kaen University

• Rattanathongkom Areeya

  Department Of Oral Biology Faculty Of Dentistry Khon Kaen University

関連論文

• Fabrication of Electrospun Thai Silk Fibroin Nanofiber and Its Effect on Human Gingival Fibroblast : A Preliminary Study
• Comparative Analysis of Gene Expression by cDNA Microarray between Cementoblasts and Periodontal Ligament Cells in the Murine Mandible

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