Response of Human Lymphocytes In Vitro to Two Dietary Furans, Furfuryl Alcohol and 2-Furyl Methyl Ketone

概要

論文の詳細を見る
The genotoxicity of furfuryl alcohol and 2-furyl methyl ketone was assessed in human lymphocytes in vitro using standard chromosome aberration analysis and sister chromatid exchange (SCE) analysis. Three doses ranging from 240 to 960 ppm which corresponded to in vivo doses were selected. Lymphocytes were cultured using TC-199 medium plus required supplements and initiated using blood drawn from healthy human donors who had not been exposed to any drugs or chemicals for a substantial period of time. After 24, 48, and 72 hr of compound exposure, slides were prepared and analyzed for variations in the mitotic index (MI) and chromosome aberrations. For SCE analysis, 10μg of 5-bromodeoxy uridine was added 24 hr after culture initiation and slides were prepared and stained using the standard technique. Two hundred metaphase spreads were scored per dose per period to analyze chromosome aberrations, and 5000 cells were randomly scored to calculate the MI. Seventy-five metaphase spreads in second mitotic divisions were scored for each dose and period for SCE analysis. All data were subjected to stastical analysis. There was an insignificant induction of chromosome aberrations and mitodepression (MD), while SCEs showed an increased incidence with both compounds which was dose and period dependent.
2008-02-01