Molecular Authentication and Quality Control Using a High Performance Liquid Chromatography Technique of Fructus Evodiae(Pharmacognosy)

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概要

• 論文の詳細を見る

• In present paper, the properties of molecular authentication combined with the fingerprints of high performance liquid chromatography (HPLC) were validated by analyzing ten batches of Fructus Evodiae samples (the dried nearly ripe fruit of Evodia rutaecarpa (JUSS.) BENTH., Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG or Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG). The results of this investigation show that the similarities of internal transcribed spacer (ITS) sequences were almost 100% in Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG, 97% in Evodia rutaecarpa (JUSS.) BENTH, and 96% in Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) HUANG. The percentage of identity between the two groups of Evodia rutaecarpa (JUSS.) BENTH. var. bodinieri (DODE) HUANG and Evodia rutaecarpa (JUSS.) BENTH. var. officinalis (DODE) UANG is almost 96%, but the identity among the group of these three species is only 73%. The results show that Fructus Evodiae comes from three species respectively. The fingerprints of HPLC show that Fructus Evodiae revealed 20 major common peaks. And the three species have almost the same chemical constituents. ITS sequence fingerprint combining the fingerprint of HPLC can not only be developed to identify and distinguish the three species in detail, but also can be used for optimizing location where Fructus Evodiae has much higher bioactive constituents and yield.

• 公益社団法人日本薬学会の論文
• 2008-02-01

著者

• Zhang Ping

  College of Pharmacy, Inner Mongolia University for the Nationalities

• Huang Dan

  Provincial Key Laboratory Of Drug Research Hunan Academy Of Traditional Chinese Medicine

• Wei Li

  College Of Life Science Hunan Normal University

• LI Shun

  Provincial Key Laboratory of Drug Research, Hunan Academy of Traditional Chinese Medicine

• CAI Guang

  Provincial Key Laboratory of Drug Research, Hunan Academy of Traditional Chinese Medicine

• YUE Chun

  Provincial Key Laboratory of Drug Research, Hunan Academy of Traditional Chinese Medicine

• Li Shun

  Provincial Key Laboratory Of Drug Research Hunan Academy Of Traditional Chinese Medicine

• Yue Chun

  Provincial Key Laboratory Of Drug Research Hunan Academy Of Traditional Chinese Medicine

• Cai Guang

  Provincial Key Laboratory Of Drug Research Hunan Academy Of Traditional Chinese Medicine

• Zhang Ping

  College Of Life Science Hunan Normal University

関連論文

• Oxygenated Hexylitaconates from a Marine Sponge-Derived Fungus Penicillium sp.
• P-475 Cytotoxic Indole Alkaloids from a Marine Sponge Spongosorites sp.
• Molecular Authentication and Quality Control Using a High Performance Liquid Chromatography Technique of Fructus Evodiae(Pharmacognosy)

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