Development of Species Specific AFLP-Derived SCAR Marker for Authentication of Panax japonicus C. A. MEYER(Molecular and Cell Biology)
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概要
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Panax japonicus is an important medicinal plant. The aim of this study was to develop species-specific molecular markers for P. japonicus. Amplified fragment length polymorphism (AFLP) was compared among P. japonicus, P. ginseng and P. quinquefolius. A clear species-specific AFLP marker for P. japonicus was generated. After isolation and sequencing of the AFLP fragment, a DNA sequence (293 bp) was obtained and named JG14. Oligonucleotide primer (23 mer) was designed for amplifying 191 by of the sequence of JG14. PCR analysis revealed a clear amplified band for P. japonicus but not in 3 other Panax species (P. ginseng, P. quinquefolius and P. notoginseng). This sequence characterized amplified regions (SCAR) marker will be used for rapid authentication of P. japonicus among other related Panax species. This is the first report of species-specific SCAR marker development in P. japonicus.
- 公益社団法人日本薬学会の論文
- 2008-01-01
著者
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YOON Eui-Soo
Department of Biology, Kongju National University
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CHOI Yong-Eui
Division of Forest Resources, College of Forest Sciences, Kangwon National University
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Kim Bo-bae
Department Of Biology Kongju National University
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Ahn Chang
Division Of Forest Resources Colleges Of Forest Sciences Kangwon National University
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Choi Yong-eui
Division Of Forest Resources Colleges Of Forest Sciences Kangwon National University
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Choi Yong-eui
Division Of Forest Resources College Of Forest Sciences Kangwon National University
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Yoon Eui-soo
Department Of Biology Kongju National University
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Choi Yong-eui
Division Of Forest Resources College Of Forest And Environmental Sciences Kangwon National Universit
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- Development of Species Specific AFLP-Derived SCAR Marker for Authentication of Panax japonicus C. A. MEYER(Molecular and Cell Biology)
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