Direct Measure of the Rate of Decay of Intermediate B in the Bleaching of Rhodopsin in the Excised Eye of the Albino Rat
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Kinetic studies on the fast photovoltage (FPV) of the intermediate B (B) in the bleaching process of the excised eye of the albino rat were performed. By using some new combinations of the blue and green test flashes the half-lives and several kinetic constants of B were obtained. Two different methods of experiments gave a similar value of the half-life of B, which is smaller by a factor of three or four than the value reported by Ebrey. The photo-regeneration of B from its daughter C was demonstrated, which explains the discrepancy.
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- Direct Measure of the Rate of Decay of Intermediate B in the Bleaching of Rhodopsin in the Excised Eye of the Albino Rat