Mucopolysaccharides from the Connective Tissues of the Squid, Ommastrephes sloani pacificus
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概要
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The mucopolysaccharides of the connective tissues, skin and cartilge of the squid (Ommastrephes sloani pacificus) were isolated and characterized. The main mucopolysaccharide component isolated from squid skin is indicated to be a non-sulfated chondroitin from the results of chemical analysis, [α]_D, electrophoresis, infrared spectrum, identification of repeating unit, measurements of hydrolysis rate of hyaluronidase digestion and formation of non-sulfated disaccharide by chondroitinase digestion. The mucopolysaccharide isolated from cartilage was found to be homogenious on electrophoresis and cetylpyridinium chloride fractionation. This substance was shown to be a new type of chondroitin polysulfate by the following evidence: 1. The ratios of uronic acid and sulfate to hexosamine were 0.97 and 1.55, respectively. 2. The infrared spectrum showed absorption bands for both axial and equatorial sulfate group. 3. The Morgan-Elson color density of hyaluronidase digests and the half lives of ester sulfate on acid hydrolysis indicated that the sulfate groups are associated not with the glucuronic acid moiety, but with the acetylgalactosamine residue at the C4 and C6 positions. 4. By chondroitinase digestion, a noval unsaturated disaccharide bearing two sulfate groups was derived as a major product, which was identified to be 2-acetamido-2-deoxy-3-O-(β-D-gluco-4-enepyranosyluronic acid)-4, 6-di-O-sulfo-D-galactose. The name "chondroitin sulfate E" was suggested for this chondroitin polysulfate containing a 4, 6-disulfated acetylgalactosamine residue.
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