RFLP Markers for the Selection of Pollination-constant and Non-astringent (PCNA)-Type Persimmon and Examination of the Inheritance Mode of the Markers

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概要

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• We investigated marker-assisted selection of the pollination constant and non-astringent (PCNA) trait and inheritance modes of the marker locus in persimmon (Diospyros kaki Thunb.) using restriction fragment length polymorphism (RFLP) analysis. In two backcross progenies, FU-170 and FU-275, 5 of 71 and 23 of 101 offspring, respectively, were judged as PCNA genotype by the marker phenotype. The astringency type of individual offspring was determined by measuring the size of tannin cells and soluble tannin content in mature fruit, thus confirming complete co-segregation of RFLP markers and the non-PCNA phenotype in 32 and 68 fruit-obtainable offspring from FU-170 and FU-275, respectively; therefore, RFLP markers can be used for PCNA genotype detection. Their segregation ratio deviated significantly from that of disomic inheritance. We also discuss the possibility of polysomic inheritance of RFLP markers.

• 園芸学会の論文

著者

• Ikegami Ayako

  Kyoto University

• Kitajima Akira

  Kyoto University

• UTSUNOMIYA Naoki

  Faculty of Agriculture, Kinki University

• Kanzaki Shinya

  Labratory of Horticultural Science, Faculty of Agriculture, Kinki University

• Yamada Masahiko

  Grape and Persimmon Research Station, National Institute of Fruit Tree Science

• Sato Akihiko

  Grape and Persimmon Research Station, National Institute of Fruit Tree Science

• Kanzaki Shinya

  Faculty of Agriculture, Kinki University

• Yonemori Keizo

  Kyoto University

• Kanzaki Shinya

  Labratory Of Horticultural Science Faculty Of Agriculture Kinki University

• Utsunomiya Naoki

  Labratory Of Horticultural Science Faculty Of Agriculture Kinki University

• Utsunomiya Naoki

  Faculty Of Agriculture Kinki University

• Yamada Masahiko

  Grape And Persimmon Research Station National Institute Of Fruit Tree Science:(present Office)kuchin

• Sato Akihiko

  Grape And Persimmon Research Station National Institute Of Fruit Tree Science

関連論文

• Network-Transparent Object Naming and Locating in the GALAXY Distributed Operating System
• Conversion of RFLP Markers for the Selection of Pollination-Constant and Non-Astringent Type Persimmons (Diospyros kaki Thunb.) into PCR-Based Markers
• Changes in Properties during Maturation and Ripening of 'Chiin Hwang No.1'Mango Fruit Cultivated in a Plastic Greenhouse
• Changes in Physical and Chemical Properties during Maturation of Mango Fruit (Mangifera indica L. 'Irwin') Cultured in a Plastic Greenhouse
• RFLP Markers for the Selection of Pollination-constant and Non-astringent (PCNA)-Type Persimmon and Examination of the Inheritance Mode of the Markers
• 遮光条件下におけるチェリモヤの葉内クロロフィル含量・葉形態・新梢生長・葉のガス交換および果実生産の日本におけるビニルハウス内での反応

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