Salinity Stress Enhances Production of Solasodine in Solanum nigrum L.

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Various in vitro grown tissues (non-regenerative callus, regenerative callus and microshoot derived leaves) of Solanum nigrum L. were cultured under salinity stress (0-150mM NaCl) for enhanced production of solasodine, a steroidal alkaloid and an alternative to diosgenin, which is used as a precursor for the commercial production of steroidal drugs. The role of plant growth regulators and various concentrations of NaCl during in vitro production of solasodine was studied. The in vitro yield was compared with the yield from leaves of field grown plant. Solasodine content was maximum (2.39mg/g dry wt.) in regenerative callus when grown on medium added with 150mM NaCl ; followed by in vitro raised leaf of microshoot. Quantitative estimation of solasodine was carried out using a new HPTLC method, which is validated for its recovery and precession. The proposed HPTLC method showed a good linear relationship (r^2=0.994) in 50-2000ng/spot concentration ranges. The data demonstrate that the solasodine production in cultures was growth dependent.
公益社団法人日本薬学会の論文
2008-01-01