Purification and Characterization of 2-Aminoacetophenone Reductase of Newly Isolated Burkholderia sp. YT(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
We found that a newly isolated Burkholderia sp. produced (R)-2-amino-1-phenylethanol from 2-aminoacetophenone, showing the high stereospecificity. NADPH-dependent 2-aminoacetophenone reductase purified to homogeneity was a dimer with a molecular mass of 65, 000. The purified enzyme did not reduce acetophenone and 1-phenyl-1-propanone. The purified enzyme converted 2-aminoacetophenone to only (R)-2-amino-1-phenylethanol.
- 社団法人日本生物工学会の論文
- 2007-11-25
著者
-
Yamada-Onodera Keiko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
Tani Yoshiki
Faculty Of Bioenvironmental Science Kyoto Gakuen
-
Yamada(Onodera) Keiko
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
Takase Yuhki
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
Yamada(onodera) Keiko
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Takase Yuhki
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
-
Tani Yoshiki
Faculty Of Agriculture Kyoto University
-
Yamada-onodera Keiko
Graduate School Of Biological Sciences Nara Inst. Of Sci. And Technol.
関連論文
- Purification, Characterization, and Gene Cloning of Glycerol Dehydrogenase from Hansenula ofunaensis, and Its Expression for Production of Optically Active Diol(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Cloning, Sequence Analysis, and Expression in Escherichia coli of Gene Encoding N-Benzyl-3-Pyrrolidinol Dehydrogenase from Geotrichum capitatum(ENZYMOLOGY, PROTEIN ENGINEERING, AND ENZYME TECHNOLOGY)
- Purification and Characterization of 2-Aminoacetophenone Reductase of Newly Isolated Burkholderia sp. YT(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Purification and Characterization of Alcohol Dehydrogenase Reducing N-Benzyl-3-Pyrrolidinone from Geotrichum capitatum(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Purification and Characterization of an Enzyme That Has Dihydroxyacetone-Reducing Activity from Methanol-Grown Hansenula ofunaensis
- Production of Optically Active 1,2,4-Butanetriol from Corresponding Racemate by Microbial Stereoinversion(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
- Comparison of Glycerol Dehydrogenases from Cellulomonas sp.NT3060 and Escherichia coli