プロテオミクス的手法を応用した消化器癌関連糖タンパク質の解析(2005年度学部等研究教育補助金採択者報告)

概要

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Cell surface glycosylation changes dramatically during oncogenesis. Among the many types of carbohydrates, sialic acid is extremely important in the development of cancer, because enhanced sialylation is thought to play an important role in tumor progression and metastasis. To investigate the clinicopathological significance of sialoglycoconjugates in various gastrointestinal cancers, we performed histochemical analysis using sialic acid-binding lectins. Primary and metastatic cancer tissues were subjected to histochemical staining using Maackia amurensis leukoagglutinin (MAL). In gastric cancers, a high level of MAL staining was significantly related to unfavorable pathological features. Next, two-dimensional polyacrylamide gel electrophoresis followed by blotting MAL-positive glycoproteins onto a PVDF membrane was performed to characterize the cancer-related sialoglycoproteins in gastric cancer. MAL-positive glycoproteins were found in samples from the cancerous region but not in those from non-cancerous region. These glycoproteins have molecular weights of approximately 200kDa or higher, and have diverse pI values probably due to the degree of sialylation. Thus, the aberrant expression of sialoglycoconjugates might play an important role in tumor progression and may be useful as one of the clinicopathological factors in various gastrointestinal cancers.
東海大学の論文