Identification and Characterization of Novel Human Recombinant Monoclonal Fab Fragments Specific for EBV Viral Capsid Antigen Established by Phage Display

スポンサーリンク

概要

• 論文の詳細を見る

• PURPOSE : Serological anti-Epstein-Barr Virus (EBV) viral capsid antigen (VCA) titer is significantly associated with malignant lymphoid proliferation. It is important to develop new sensitive method to detect EBV. The purpose of this study is to generate specific EBV-VCA human monoclonal antibodies. MATERIALS AND METHODS : By utilizing a phage display system we constructed a human antibody library from a patient with Sjogren's syndrome (SS) carrying high serum anti-EBV VCA titer. Four Fab fragments (Fab1, Fab15, Fab16 and Fab21) were isolated by panning Fab library against EBV-VCA protein coated onto ELISA plates. Selected Fab clones were characterized by ELISA, inhibition ELISA, Western blotting (WB), indirect immunofluorescence assay (IFA) and immunohistochemistry. RESULTS : We found that two (Fab1 and Fab21) of four Fab clones specifically bound to EBV-VCA by ELISA and WB study. Inhibition ELISA competition revealed that these two Fab clones could significantly reduce the binding of specific anti-EBV-VCA mouse monoclonal antibody to its relevant proteins. Furthermore, these two Fabs can be used for the detection of VCA protein in EBV-infected P3HR1 cell line and tissue samples from patients with EBV-related malignancies. Whereas the other two Fab clones (Fab15 and Fab16) did not indicate these specificities. CONCLUSION : These results indicate that our two Fab clones (Fab1 and Fab21) are novel human monoclonal antibodies which are specific for EBV-VCA protein and may have potential benefits for development of novel diagnostic and therapeutic approaches in EBV-related lymphoid malignancies.

• 金沢医科大学の論文

著者

• DONG Lingli

  Division of Hematology and Immunology, Department of Internal Medicine, Kanazawa Medical University

• Dong Lingli

  Division Of Molecular Oncology And Virology Kanazawa Medical University:department Of Hematology And

関連論文

• Fractalkine in rheumatoid arthritis and allied conditions
• Identification and Characterization of Novel Human Recombinant Monoclonal Fab Fragments Specific for EBV Viral Capsid Antigen Established by Phage Display

スポンサーリンク