Flocculation of Saccharomyces diastaticus Grown with Starch

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概要

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• Cells of Saccharomyces diastaticus IFO 1958 did not flocculate 24h after inoculation in the medium with starch as carbon source but flocculated 48h after inoculation. Cycloheximide completely inhibited induction of floc-forming ability of cells grown for 24h in the medium including starch. The effect of chemical modification of cell surface protein and carbohydrate components on the floc-forming ability of flocculent cells grown with starch for 48h and72 h was studied. Treatment with proteolytic enzymes destroyed the floc-forming ability. Photo-oxidation in the presence of methylene blue and reduction with mercaptoethanol eliminated the flocculent ability. Oxidation with sodium periodate also deprived the cells of floc-forming ability. High concentrations of protein-denaturants brought about reversible deflocculation of the flocculent cells. These findings suggest that cell surface protein and carbohydrate components play essential roles in flocculation of the cells grown with starch.

• 香川大学の論文
• 2006-09-30

著者

• 三宅 舞

  Department Of Chemistry Faculty Of Education Kagawa University

• 西原 浩

  Department of Chemistry, Faculty of Education, Kagawa University

関連論文

• Flocculation of Saccharomyces diastaticus Grown with Starch

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