ECP Levels in Nasal Mucus Depend on Eosinophil Collapse

概要

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Objective : Degranulation of eosinophils is believed to cause epithelial failure. Some researchers have believed that degranulation of eosinophils occurs in the form of piecemeal degranulation (PMD), while others have reported that breakdown of the eosinophil cell membrane causes degranulation. In this study, I examined by which form the eosinophil granule proteins in nasal mucus are mainly released from eosinophils.Methods : Nasal mucus collected by suction were observed by electron microscopically. Total eosinophils, collapsed eosinophils, and vacuolated granules were counted. Destruction of eosinophil cell membrane was classified into 4 ranks by degree of failure of the cell membrane. Granule vacuolation was classified into 3 ranks based on granule properties. A destruction index was defined as the product of the sum of granule vacuolation score and the score for destruction of eosinophil cell membrane. The ECP level in lavage fluid from the nasal cavity was also measured.Results : The ECP level in lavage fluid and score for destruction of eosinophil cell membrane were significantly correlated (R = 0.813, p = 0.0077), while ECP level and destruction index which was derived from both the destruction of cell membrane and granule vacuolation exhibited an even stronger correlation (R = 0.988, p < 0.0001).Conclusion : The eosinophil granule proteins in nasal mucus were released mainly from collapsed eosinophils.
獨協医科大学の論文
2006-10-25