Antioxidant Evaluation of Eysenhardtia Species (Fabaceae) : Relay Synthesis of 3-O-Acetyl-11α,12α-epoxy-oleanan-28,13β-olide Isolated from E. platycarpa and Its Protective Effect in Experimental Diabetes(Pharmacognosy)

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概要

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• The antioxidant activities of plant extracts from Eysenhardtia platycarpa, E. punctata, and E. subcoriacea (Fabaceae) species, used in Mexican traditional medicine for the treatment of diabetes complications, were analyzed in a rat pancreas homogenate model. Methanolic extracts of E. platycarpa, E. punctata, and E. subcoriacea protected the pancreatic homogenate from 2,2-azo-bis(2-amidinopropane)dihydrochloride (AAPH)-induced damage. The inhibitory effect was dose-dependent at concentrations of 10-1000ppm and correlated with 1,1 diphenyl-2-picrylhydrazyl (DDPH) radical scavenger capacity. 3-O-Acetyl-11α,12α-epoxy-oIeanan-28,13β-olide (1, EC_<50>=21.2±2.2 μM), (+)-catechin (2, EC_<50>=7.4±1.1 μM), and (+)-catechin 3-O-β-D-galactopyranoside (3, EC_<50>=11.5±1.5 μM), natural constituents isolated from the branches of E. platycarpa, displayed significant antioxidant activity. Compounds 1 and 2 significantly increased (p<0.001) the pancreatic glutathione (GSH) concentration alone and in combination with AAPH treatment. Compound 1 was obtained from oleanolic acid by relay synthesis via acetylation, bromo-lactonization, dehydrobromination, and oxidation, and its antioxidant effect was evaluated on streptozotocin (STZ)-induced diabetic rats. On its own, 1 at a dose of 100 mg/kg b. wt. (i.p.) for 5 d significantly increased the activities of glutathione peroxidase (GSHPx) and catalase (CAT). Simultaneous treatment of 1 (100 mg/kg b. wt.) and STZ significantly reduced the pancreatic thiobarbituric acid reactive substances (TEARS) concentration together with a significant increase in the activities of GSHPx and CAT, preventing hyperglycemia induced by STZ after 5 d of treatment. The present study demonstrates the antioxidant and antihyperglycemic activities of compound 1 isolated from Eysenhardtia species used in traditional medicine.

• 公益社団法人日本薬学会の論文
• 2007-08-01

著者

• Soto Claudia

  Departamento De Sistemas Biologicos Universidad Autonoma Metropolitana-xochimilco

• NARVAEZ-MASTACHE Jose

  Institute de Quimica de la Universidad Nacional Autonoma de Mexico, Circuito Exterior, Ciudad Univer

• DELGADO Guillermo

  Institute de Quimica de la Universidad Nacional Autonoma de Mexico, Circuito Exterior, Ciudad Univer

• Delgado Guillermo

  Institute De Quimica De La Universidad Nacional Autonoma De Mexico Circuito Exterior Ciudad Universi

• Narvaez-mastache Jose

  Institute De Quimica De La Universidad Nacional Autonoma De Mexico Circuito Exterior Ciudad Universi

関連論文

• Antioxidant Evaluation of Eysenhardtia Species (Fabaceae) : Relay Synthesis of 3-O-Acetyl-11α,12α-epoxy-oleanan-28,13β-olide Isolated from E. platycarpa and Its Protective Effect in Experimental Diabetes(Pharmacognosy)

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