Application of polyelectrolyte hydrogel in microfluidic chips (Silicon devices and materials: 第15回先端半導体デバイスの基礎と応用に関するアジア・太平洋ワークショップ(AWAD2007))

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Unique characteristics of polyelectrolyte offer various functionalities to microfluidic chips for analysis and manipulation. Since polyelectrolytes bear electrolyte units, their solutions are electrically conductive. They can be simply coated or built into structures in microfluidic channels by in-situ photopolymerization. One of the applications is an electrochemical cell with a stable reference electrode unit. A thin layer cell was constructed in a PDMS chip using at polyelectrolyte salt bridge, poly diallyldimethylammonium chloride (pDADMAC). The novel polyelectrolyte reference electrode showed reproducibly 19±6mV vs. commercial Ag/AgCl reference electrode and maintained, the potential over 30 hours. Differential pulse voltammetry (DPV) was carried out in the electrochemical cell and clearly detected 5μM dopamine. Current peak linearly proportional to the concentration of dopamine and the limit of detection was calculated to 2μM. The salt bridge could be applied to cell electroporation in microfluidic chips. Our electroporation microchip precisely controls the electric field on cells without bubble generation in microchannel by virtue of the ionic conductivity of polyelectrolyte hydrogel. According to the calculation with CFD-ACE, the electric field across the microchannel is around 0.9 kV/cm only with 10 V input voltage meeting the requirements for electropermeation (0.3〜1kV/cm). Electropermeation of K562 human chronic leukemia cells was accomplished in the microchip from 5V and the efficiency increased up to 60% with viability of 80% at 15V. Transfection and expression of DNA plasmid was also successfully demonstrated in the suspension cell line.
社団法人電子情報通信学会の論文
2007-06-18

Application of polyelectrolyte hydrogel in microfluidic chips (Silicon devices and materials: 第15回先端半導体デバイスの基礎と応用に関するアジア・太平洋ワークショップ(AWAD2007))

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