Atomic Force Microscopy Observation of the Phase Separation in Acidic Phospholipid/Neutral Phospholipid Supported Bilayers

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概要

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• Phase separation induced by Ca^<2+> in the DPPS (dipalmitoyl phosphatidylserine)IDPPC (dipalmitoyl phosphatidyicholine) or DPPSIDOPC (dioleoyl phosphatidyicholine) system was observed by atomic force microscopy (AFM). Lateral modulation frictional force microscopy (LM-FFM) revealed phase separation in supported bilayer membranes prepared by the transfer of the monolayers of DPPS/DPPC with a small amount of Ca^<2+> in the subphase, while the phase separation was not shown in their topographic images obtained by contact mode AFM. Phase separation induced by Ca^<2+> in DPPSIDOPC mixed systems was observed in topographic images and the time course of the appearance and development of DPPSIDOPC (1/9) phase separation after the addition of Ca^<2+> (0.1 mM) was revealed.

• 社団法人応用物理学会の論文
• 2002-07-30

著者

• Wakayama Nobuyuki

  Faculty Of Engineering Tom University Of Yokohama

• TAGUCHI Shuichi

  Faculty of Engineering Tom University of Yokohama

関連論文

• Formation Mechanism of Multilayer Polyamic Acid Alkylamine Salt Langmuir-Blodgett Films
• Analysis of Microstructure of Polyamic Acid Alkylamine Salt Monolayers by Atomic Force Microscopy and Friction Force Microscopy
• Atomic Force Microscopy Observation of the Phase Separation in Acidic Phospholipid/Neutral Phospholipid Supported Bilayers

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