ラットにおける正常細胞と白血病細胞のDNA複製パターンの解析
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概要
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Differential staining method for detection of DNA replicating regions in rat metaphase chromosomes was improved by adding urea treatment to the differential Giemsa method of sister chromatids. Using this BrdU-Hoechst 33258-Light-Urea-Giemsa technique, the late replicating chromosomal regions of normal bone marrow cells were shown to be on #1, 2, 3, 4, 11, 12, 13, 19 and Y chromosomes, with excellent resolution. Comparative analysis of the late replicating regions of 9 in vitro erythroblastic leukemia lines established from 7, 12-dimethylbenz (a) anthracene (DMBA)-and N-butyl-N-nitrosourea(BNU)-induced primary leukemias, were carried out. In some leukemia lines, difference was not recognized in the time schedule of DNA replication as compared to normal bone marrow cells, but several abnormal late replicating regions were detected, especially, in #2 trisomy and marker chromosomes involving #1,2,3 and #12 chromosomes. The role of these late replicating regions in the origin of reproducible chromosomal changes in DMBA- and BNU-induced primary rat leukemias and in the secondary chromosomal changes during leukemia cell proliferetion was discussed.
- 神戸大学の論文