HNF-1 Regulates the Promoter Activity of the HP-27 Gene(SHORT COMMUNICATION,Molecular Biology)
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概要
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The hibernation-specifics HP-27 gene is expressed specifically in the liver of the chipmunk, a hibernating species of the squirrel family, and exists as pseudogene in the tree squirrel, a nonhibernating species. In the promoter region, the chipmunk gene has a potential HNF-1 binding site, and the tree squirrel gene has two base substitutions in the corresponding sequence. In this paper, we investigated the role of HNF-1 in the HP-27 gene promoter activity. gel retardation assays with in vitro-translated HNF-1 and super-shift assays using HepG2 nuclear extracts and an anti-HNF-1 antibody revealed that HNF-1 bound to the chipmunk gene sequence. HNF-1 also bound to the tree squirrel sequence, but with much lower affinity. In HepG2 cells, HNF-1 activated transcription from the chipmunk HP-27 gene, but not from the tree squirrel gene. In addition, the tree squirrel-type base substitutions in the HNF-1 binding site greatly reduce the promoter activity of the chipmunk HP-27 gene. These results indicate that HNF-1 is required for the promoter activity of the chipmunk HP-27 gene, and that the base substitutions in the HNF-1 binding site are involved in the lack of HP-27 gene expression in the tree squirrel.
- 社団法人日本動物学会の論文
- 2004-04-25
著者
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SHIBA Tadayoshi
Department of Molecular Biology, Kitasato University
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TAKAMATSU Nobuhiko
Department of Bioscience, School of Science, Kitasato University
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ITO Michihiko
Department of Bioscience, School of Science, Kitasato University
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Shiba Tadayoshi
Department Of Bioscience School Of Science Kitasato University
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Kojima-kawagoe Miho
Department Of Biosciences School Of Science Kitasato University
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Ono Motoharu
Department Of Biosciences School Of Science Kitasato University
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Kondo Noriaki
Mitsubishi Kasei Institute of Life Science
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Ito Michihiko
Department Of Bioscience School Of Science Kitasato University
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Ito Michihiko
Molecular Biology Department Of Biosciences School Of Science Kitasato University
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Takamatsu Nobuhiko
Department Of Bioscience School Of Science Kitasato University
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Kondo Noriaki
Mitsubishi Kagaku Institute Of Life Sciences
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TAKAMATSU Nobuhiko
Department of Biophysics and Biochemistry, Faculty of Science, University of Tokyo
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