奇形を伴う実験的染色体異常症誘発の試み

概要

論文の詳細を見る
Mutagens. 7, 12-dimethylbenz(a)anthracene (DMBA) and ethyl methanesulfonate (EMS). were injection into male Wistar rats, and they were mated with normal females 7-46 days after the treatment. DMBA prevented differentiation of spermatogonia to primary spermatocytes for 8 weeks and gave lethal damage to primary spermatocytes, but no detectable change was observed in the EMS group. Dominant lethals were induced when male cells were treated with DMBA at speramatid(54.8%) and primary spermatocyte (73.2%) stage and with EMS at spermatid stage (96.4 %). Malformed fetuses were induced only when germ cells were treated at the spermatid stage. Eight malformed 23 fetuses were obtained among 1037 live fetuses (0.77%) in DMBA group(25mg/kg) and one among fetuses in EMS (100mg/kg) group. They consisted of otocephaly, polydactylia, syndactylia, clubfoot, phocomelia, shortening of arms and legs and defect of vertebrae. In 2 of 8 cases chromosome changes were found with cytogenetical analysis. Only 2 cases with external malformations, exencephaly and micrognathia, were detected among 1220 control fetuses.
神戸大学の論文