Down症候群患者の培養リンパ球における姉妹染色分体交換に関する研究

概要

論文の詳細を見る
The Giemsa technique for Sister Chromatid Exchanges (SCE) was used to study the incidence and the distribution of SCE of lymphocyte from Down's syndrome patients and normal controls. The following results was offered : (1) The frequency of SCE was dependent on concentration of 5-Bromodeoxyuridine (BrdU) in the culure medium at the levle of higher than 7.5μg/ml, but was not influenced at the level of 2μg/ml. (2) The mean spontaneous SCE in lymphocytes in normal controls was 5.5 per cell, and was independent of sex and age. (3) The mean spontaneous SCE in lymphocytes in Down's syndrome patients was 10.2 per cell, which was significantly higher than normal controls. (4) The inter-chromosomal distribution of SCE was proportional to the chromosome length although the smaller chromosome had fewer SCE than expected. No.1 and No.2 chromosomes had significantly higher incident SCE in the central region of long arms. (5) Inter-chromosomal distribution of SCE in Down's syndromes had a similar tendency, No.1 chromosome revealed a higher incidence. The distribution with No.1 and No.2 chromosomes had almost the same as that in normal controls. (6) The incidence and distribution of 7, 12-dimethylbenz(a)anthracene (DMBA) induced SCE were also studied. The lymphocytes from Down's syndromes were shown to be more sensitive than those from normal controls. These result indicates that the DNA in Down's syndromes lymphocytes is more vulnerable to DMBA than in normal lymphocytes from unknown reseason and this may explaine the higher sesceptibility of Down's patients to malignancies.

Down症候群患者の培養リンパ球における姉妹染色分体交換に関する研究

スポンサーリンク

概要

著者

関連論文

スポンサーリンク