Detection of Anti-ribonucleic Acid Antibodies in Hyperthyroid Patients' Sera by a Solid-Phase Enzyme Immunoassay(Analytical,Chemical)
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概要
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A solid-phase enzyme immunoassay for the measurement of anti-ribonucleic acid (RNA) antibodies in the sera from hyperthyroid patients was developed. Three antigens, calf liver RNA, poly (I)・(C) and poly (A)・(U), were fixed to 96-well enzyme immunoassay microtiter plates precoated with poly-L-lysine hydrobromide. Antibodies detected in the present assay cross-reacted with neither double strand-deoxyribonucleic acid (ds-DNA) nor single strand (ss)-DNA, and were RNA-specific. In twenty (66.6%) out of 30 sera from hyperthyroid patients, anti-RNA antibodies were detected. Interestingly, in seven positive cases out of 8 patients with malignant thyrocele, the levels of antibodies against poly (I)・(C) and poly (A)・(U) were much higher than those against calf liver RNA, while antibodies against calf liver RNA were commonly high in hyperthyroid patients. The present assay requires no radioactive materials, and is sensitive and specific. It is expected to be practically useful to measure anti-RNA antibodies. Furthermore, this assay may provide an aid to diagnose the disease type, e.g., autoimmune or nonautoimmune, and the degree of the hyperthyroidosis.
- 公益社団法人日本薬学会の論文
- 1987-11-25