Double Transfection into Primary Dissociated Neurons(CELL AND TISSUE ENGINEERING)

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概要

• 論文の詳細を見る

• Synapses play a fundamental role in the processing of information in a neuronal network by mediating communication between neurons. To monitor presynaptic and postsynaptic structures simultaneously in living neurons, we developed double transfection method on the basis of calcium phosphate coprecipitation and introduced two fluorescent reporters into cultured neurons. The optimized protocol allowed different populations of neurons to be visualized by different color fluorescences, and presynaptic and postsynaptic structures to be observed in the same field of view of a microscope.

• 公益社団法人日本生物工学会の論文
• 2007-05-25

著者

• Yamaguchi Shun

  Division Of Molecular Brain Science Department Of Molecular Brain Sciences Kobe University Graduate

• Yamaguchi Shun

  Division Of Molecular Brain Science Department Of Brain Sciences Kobe University Graduate School Of

• Eguchi Megumi

  Division of Molecular Brain Science, Department of Molecular Brain Sciences, Kobe University Graduat

• Eguchi Megumi

  Division Of Molecular Brain Science Department Of Molecular Brain Sciences Kobe University Graduate

関連論文

• Altered food-anticipatory activity rhythm in Cryptochrome-deficient mice
• Double Transfection into Primary Dissociated Neurons(CELL AND TISSUE ENGINEERING)

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