Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus(Aphelenchida: Aphelenchoididae), at heat-shock protein 70A locus and the field detection of polymorphic populations

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概要

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• Polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP) pattern of heat-shock protein 70A gene (hsp70A) was examined for 29 isolates of the pinewood nematode. Among them, two known avirulent and five virulent isolates generated banding patterns identical within each of the two isolate groups but distinctive between the two. On the other hand, field populations were collected in two geographical areas, around Kasama City, Ibaraki Prefecture and the southern part of Kujukurihama coast, Chiba Prefecture, Japan. PCR-RFLP image analysis revealed that some of the populations possessed both of the two alleles. The geographical distribution of the alleles was random in the two sampling areas. On the basis of the results, we hypothesize that the avirulent strain might have a certain contribution to the gene pool of the pinewood nematode, although there has been no report on the collection of an avirulent population from Japan recently.

• 日本応用動物昆虫学会の論文
• 2007-05-25

著者

• Takemoto Shuhei

  Graduate School Of Agriculture Kyoto University

• Futai Kazuyoshi

  Graduate School Of Agriculture Kyoto University

関連論文

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• PCR-RFLP image analysis : a practical method for estimating isolate-specific allele frequency in a population consisting of two different strains of the pinewood nematode, Bursaphelenchus xylophilus (Aphelenchida : Aphelencoididae)
• Polymorphism of Japanese isolates of the pinewood nematode, Bursaphelenchus xylophilus(Aphelenchida: Aphelenchoididae), at heat-shock protein 70A locus and the field detection of polymorphic populations
• Making headway in understanding pine wilt disease : What do we perceive in the postgenomic era?

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