ヒト単核白血球細胞培養における黄熱ウイルス感染の生物学的特長
スポンサーリンク
概要
- 論文の詳細を見る
Interaction of yellow fever virus (YFV) and human mononuclear leucocyte cultures (J-111 line) in vitro was investigated. The results obtained were as follows. 1) Persistent infection of YFV in J-111 cells was established easily. 2) The persistently infected J-111 cells (termed J-YF in this study) resisted to challenge with dengue virus or chikungunya virus. It was assumed that the resistance as observed was not due to interferon or related anti-YFV factor(s) present in the culture fluid. 3) It was not possible to remove the YFV from the J-YF cells by applying anti-YFV rabbit IgG or by ultraviolet light irradiation. 4) The J-YF cells lost the ability in forming turnors in athymic nude mice (BALB/c AJclnu/nu). 5) When the YFV-infected J-111 cells were stained by feulgen method, cells containing feulgenpositive substances (FPS) in the nuclei significantly increased in number, compared with those in uninfected control cultureR as well as in cultures infected with dengue virus. 6) The DNA contents of FPS measured by microspectrophotometric(MSP) techniques decreased during incubation at 37℃ both in the YFV-infected and control non-infected cultures. However, the DNA in YFV-infected cells increased transitorily at 4-5 days after the virus inoculation. 7) Incorporation of 3^H-thymidine into YFV-infected J-111 cells was shown to parallel the MSP-determined DNA changes as described above.
- 神戸大学の論文