GENE EXPRESSION PROFILE IN HUMAN CELLS EXPOSED TO ZINC
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概要
- 論文の詳細を見る
Although Zn is an essential trace metal for humans, a comprehensive view of its effects on cellular functions has not been obtained. We used a DNA microarray to assess transcriptional alterations in human HeLa cells after exposure to a moderate concentration of Zn (100μM ZnSO_4). Out of 9,182 human genes, expression was increased in 7 genes and decreased in 4 genes twofold or greater. Four of the 7 upregulated genes were those coding for metallothionein isoforms or related proteins. An unexpectedly small extent of changes in gene expression might reflect rapid sequestration of Zn ions by metallothioneins, and the absence of most of the other protective responses indicated the non-toxic nature of Zn at this concentration. Comparison with our previous DNA microarray results for 5 μM CdSO_4-exposed HeLa cells revealed several genes that are regulated by both metals in parallel, and a gene reciprocally regulated by them.
- 日本トキシコロジー学会の論文
- 2007-05-16
著者
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Koizumi Shinji
Human Engineering And Risk Management Res. Group National Inst. Of Occupational Safety And Health
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Koizumi Shinji
Mechanism Of Health Effect Research Group National Institute Of Occupational Safety And Health
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Yamada Hirotomo
Mechanism Of Health Effect Research Group National Institute Of Occupational Safety And Health
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Suzuki Kaoru
Mechanism Of Health Effect Research Group National Institute Of Occupational Safety And Health
関連論文
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- GENE EXPRESSION PROFILE IN HUMAN CELLS EXPOSED TO ZINC
- Inhibition of Metallothionein Induction by Ultraviolet Light Irradiation (Proceedings of the 20th Symposium on Toxicology and Environmental Health)