Regulation of Proliferation and Steroidogenesis in Human Granulosa Cells

概要

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Background and Aims : Human follicles selected for further development are thought to receive precisesignals from gonadotropins and locally produced growth factors for survival. In this experiment, themechanisms of the regulation of proliferation and steroidogenesis by GDF-9 and Br-cAMP in humanovarian cells were investigated. Methods : SVOG cells were cultured and treated with GDF-9 andBr-cAMP. The expression of HOXA7 and cofactors were examined by Western blot analysis and/orRT-PCR. The cell proliferation was measured by thymidine incorporation assay. Results : Treatmentwith GDF-9 resulted in a significant increase of cell proliferation. Although the treatment withBr-cAMP increased the expression of StAR protein, this treatment resulted in a significant decrease of cellproliferation compared with the control and no change in HOXA7 or its cofactors. Conclusion : Thepresent study demonstrated that GDF-9 had a stimulatory effect and cAMP had an inhibitory effect onthe proliferation of SVOG cells. These results may reflect the characteristics of this human cell line asluteinized granulosa cell. Therefore, this culture system will be useful for studying the effects of localfactors and gonadotropin on the regulation of human luteinized granulosa cells in the future.
北関東医学会の論文
2007-05-01