Direct DNA Isolation from Solid Biological Sources without Pretreatments with Proteinase-K and/or Homogenization through Automated DNA Extraction(METHODS)

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概要

• 論文の詳細を見る

• Genomic DNA from solid biomaterials was directly isolated with an automated DNA extractor, which was based on magnetic bead technology with a bore-mediated grinding(BMG) system. The movement of the bore broke down the solid biomaterials, mixed crude lysates thoroughly with reagents to isolate the DNA, and carried the beads to the next step. The BMG system was suitable for the mechanical homogenization of the solid biomaterials and valid as an automated system for purifying the DNA from the solid biomaterials without the need for pretreatment or disruption procedures prior to the application of the solid biomaterials.

• 社団法人日本生物工学会の論文
• 2007-03-25

著者

• Ki Jang-seu

  Department Of Biology The Hong Kong University Of Science & Technology

• Ki Jang-seu

  Department Of Life Science College Of Natural Sciences Hanyang University

• Chang Ki

  Bionex Inc., #421 Hanyang Institute of Technology, Hanyang University

• Roh Hee

  Bionex Inc., #421 Hanyang Institute of Technology, Hanyang University

• Lee Bong

  Bionex Inc., #421 Hanyang Institute of Technology, Hanyang University

• Yoon Joon

  Mechanical Engineering Department, Hanyang University

• Jang Gi

  Bionex Inc., #421 Hanyang Institute of Technology, Hanyang University

• Roh Hee

  Bionex Inc. #421 Hanyang Institute Of Technology Hanyang University

• Jang Gi

  Bionex Inc. #421 Hanyang Institute Of Technology Hanyang University

• Jang Gi

  Bionex Genome Research Institute #421 Hanyang Institute Of Technology Building Hanyang University

• Yoon Joon

  Mechanical Engineering Department Hanyang University

• Chang Ki

  Bionex Inc. #421 Hanyang Institute Of Technology Hanyang University

• Lee Bong

  Bionex Inc. #421 Hanyang Institute Of Technology Hanyang University

関連論文

• Direct DNA Isolation from Solid Biological Sources without Pretreatments with Proteinase-K and/or Homogenization through Automated DNA Extraction(METHODS)
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• Integrated Method for Single-Cell DNA Extraction, PCR Amplification, and Sequencing of Ribosomal DNA from Harmful Dinoflagellates Cochlodinium polykrikoides and Alexandrium catenella

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