Epigallocatechin-3-O-gallate As a Novel Preservation Additive for Tooth Transplantation

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The effects of green tea polyphenol, epigallocatechin-3-O-gallate (EGCG), on tooth preservation of guinea pig incisors were investigated. Carefully extracted and pulpectomized teeth of the guinea pigs (200-300g, 4 weeks) were irrigated with saline. After rinsing in phosphate buffer saline containing 100U/mL of penicillin and 100 μg/mL of streptomycin, the teeth were transferred to Dulbecco's Modified Eagle's Medium (DMEM) containing 10 v/v% of fetal calf serum and different concentration of EGCG, followed by incubation at 4℃ for one week. Then, the teeth were recovered and put on a culture dish with DMEM. Time until cell migration of EGCG treated teeth was shorter than that of untreated teeth. The periodontal ligament cells viability preserved with 250 μg/mL EGCG at 4℃ was higher than that without EGCG. These results suggested that EGCG could be useful as a cell protective agent at low temperature preservation.
2006-03-30