生育パターンの異なる低温性Vibrio属細菌の生育に伴う菌体タンパク質の変動
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In order lo study whether the psychrotrophic Vibrio isolates showing different growth curves are composed of different ecotype, cellular proteins of the non-halophilic and halophilic psychrotrophs during growth were determined by two-dimensional gel electrophoresis using isoelectric focusing in one dimension and sodium dooecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) in the second dimension. Growth curves of the psychrotrophs showed an increased slope throughout growth (in Type 1 strains) or a decreased slope after the stationary phase of growth (in Type 2 strains). In cellular proteins of the non-halophiles, bands on both isoelectric focusing gels and SDS gels and spots on the two-dimensional gels were very clear for Type 1 strains and unclear (become foggy) for Type 2 strains, suggesting that the protein molecules have compact structure in Type 1 strains and deformable structure in Type 2 strains. In the halophiles, however. the protein bands and spots were clear for both Type 1 and 2 strains, suggesting that the molecules have compact structures for both Types of the halophiles. In the non-halophiles, the number and amounts of the protein spots increased in Type 1 strains during growth and decreased markedly in Type 2 strains during the death phase of growth. Moreover, the protein profiles obtained by the two-dimensional gel electrophoresis were very different between CBB and silver stainings and more proteins were observed in CBB staining in Type 1 strains and in silver staining in Type 2 strains of the non-halophiles. From these results, the difference of growth curves between Type 1 and 2 strains in the non-halophiles was considered to be based on the difference of physical and chemical structures for tertiary folding of protein molecules. In other words, it was suggested that the two Type strains of the non-halophilic psychrotrophic Vibrio isolates are composed of different ecotype.
- 長崎大学の論文
長崎大学 | 論文
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