HbA_1分画測定と臨床的意義に関する研究
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概要
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The present study aimed to be clarified the clinical evaluation of HbA_1 and HbA_<1C> in normal and abnormal metabolci disorders such as diabetes and renal failure. An improved HbAI determination method was developed by high-performance liquid chromatography using phosphate buffers of pH 6.65 and 6.45 without KCN. The results were as follows : 1) In diabetic patients, the greater part of HbA_1 was contained HbA_<1C>, while the less in normal subjects. 2) There was no significant difference between HbA_1 and HbA_<1C> values as one of the indicators for retrospective long-term blood glucose control during diabetic treatment. 3) HbA_<1C>/HbA_1 ratio was observed to increse parallel with the increment of HbA_1 in diabetics. 4) In the treatment of diabetic ketoacidosis using an artificial pancreas apparatus, a rapid decreased change in the part of HbA_<1C> component was observed during the period of good glucose control. 5) HbA_1 was a lot of amount in diabetes with severe sequelae, although no significance in HbA_<1C>/HbA_1 ratio was observed. 6) Not only HbA_<1C> but also HbA_<1a+b> components were increased in patients with renal failure. 7) It was demonstrated in vitro that modified hemoglobin was formed by aldehyde's derivatives, i. e. glyceraldehyde, pyridoxal HCl., etc. and also an increased amount of both HbA_<1C> and HbA_<1a+b> was involved. It was suggested that the increment of HbAI in patients with renal failure might be the results in a binding of some substances other than glucose. 8) HbA_<1C>/HbA_1 ratio was shown higher levels in the older than that in the younger erythrocytes.
- 神戸大学の論文