Rapid and Convenient Detection of Urinary Equol by Thin-Layer Chromatography

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概要

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• Equol, a metabolite of daidzein for some intestinal microflora, is known to retain highly estrogenic activity and is of wide interest in relation to human health. However, not all humans can produce equol. In this study, detection of urinary equol using thinlayer chromatography (TLC) was performed to distinguish between equol producers and non-producers. After 36 h of soy food intake, urine, collected from 7 volunteers, was hydrolyzed, purified by reverse phase silica gel column and applied to normal phase TLC. Consequently, equol was clearly separated from the urine samples and discriminated the equol producers in this system. The detection limit of equol was at least 20 ng.

• 日本ビタミン学会の論文

著者

• Yokoyama Shin-ichiro

  Research Institute For Bioengineering Gifu Prefectural Government

• KUZUGUCHI Tsuyoshi

  Research Institute for Bioengineering, Gifu Prefectural Government

• Kuzuguchi Tsuyoshi

  Research Institute For Bioengineering Gifu Prefectural Government:(present Address)research And Deve

関連論文

• Rapid and Convenient Detection of Urinary Equol by Thin-Layer Chromatography

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