アミラーゼインヒビターの基礎的検討並びに臨床的応用
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概要
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The mode of inhibitory actions of a-amylase inhibibitors extracted from culture broth of Actinomyces (α-glucosidehydrolase inhibitor : α-GHI) and wheat, and the clinical usefulness of these inhibitors have been evaluated. Both inhibitors had a non-competitive type of inhibitory actions on human α-amylase activity. α-GHI inhibited pancreatic α-amylase more potently than salivary α-amylase, whereas α-amylase inhibitor extracted from wheat had a specific inhibitory action on salivary α-amylase. Column chromatographic investigations suggested the possible formation of an enzyme-inhibitor complex, which was easily separated into each component after electrophoresis and the enzyme activity was fully recovered. α-amylase inhibitor extracted from wheat can be used to determine the ratio of pancreatic to salivary type isoamylase activity, because of its specific inhibitory action on salivary a-amylase. There was good correlation in P/S ratio of serum from 25 healthy subjects between the results obtained with inhibitor method and those obtained using electrophoresis. This method would be widely applied in clinical practice for performing isoamylase analysis because of its technical simplicity and analytical rapidity. Because α-GHI is not only an a-glucosidase inhibitor but inhibits also a-amylase, oral administration of α-GHI reduced the postprandial plasma glucose rise in healthy subjects. α-GHI may be useful in the treatment of carbohydrate dependent diseases to limit intestinal carbohydrate digestion.
- 神戸大学の論文