歯周疾患に罹患した歯根面とその改善に関する研究

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概要

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The purpose of this study is to observe the denuded root surfaces involving in periodontal disease, and to investigate the biological compatibility of them which were treated by representative clinical procedures. 145 periodontally diseased human teeth and 25 normal undenuded teeth were used in this experiment. The surfaces and cross sections of these roots were examined by scanning electron microscope and micro-radiography. We also made clinical treatment on periodontally involved root such as scaling, root planing, complete dentin exposing and some of them were subjected by citric-acid application. Then small root fragments of these treated teeth were incubated in culture medium with fibroblasts derived from healthy human gingiva. After 2 days of incubation, root fragments with cells were fixed and stained. The number of cells on each sample was calculated using light microscope. The results were followed: a. SEM observation indicated that the denuded root surfaces were covered by hypermineralized zone which showed acid resistance. 2. This hypermineralized zone showed radiopasity under microradiographic observation. The thickness of this zone was the widest at cervical area and gradually narrowed to bottom of periodontal pocket. 3. The growth of cells on treated root surfaces was increased according to remove the contaminated and hypermineralized cementum. 4. After application of citric-acid to root surfaces, the cell growth was most remarkable on root planed tooth. However on scaled tooth with acid appliation, the number of cells was not different from tooth without acid application This experiment suggested that removal of hypermineralized zone from denuded root improve the tooth root surfaces which involving in periodontal contamination. Citric-acid application after mechanical remove of them would faciliate the gingival reattachment to treated root surfaces in this in vitro study.
1982-03-28

著者

濱久友
北海道大学歯学部歯科保存学第2講座

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