歯周ポケット滲出液中の燐脂質量の変動に関する研究 : とくにホスファチジルコリン,ホスファチジルエタノールアミン,スフィンゴミエリン量について

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The formation of periodontal pockets is generally accepted as one of the three symptoms of a chronic marginal periodontitis. The exudation which flows itself into these periodontal pockets increases proportionately to the aggravation of a disease. There have been published many research reports on the composition of this periodontal pocket exudate to date, including those by Brill. As for lipid substance in the exudate, however, the published literature is scanty except for that of Watanabe among the domestic investigators. Further, concerning the phospholipids there seems to be a qualitative report by the present author and his collaborators alone. For this reason, the present study was concerned with the classification of the degrees of oral hygiene, gingival inflammation and depth of periodontal pockets on the part of patients suffering from chronic marginal periodontitis, efforts being made to measure quantitatively the values of phosphatidylcholin, phosphatidylethanolamine and sphingomyelin for the purpose of comparative analysis with the clinical findings. By way of study material, a total of 54 patients (26 males and 28 females) who visited the Department of Periodontology, Nihon University Dental Hospital, were selectively used. The subjects, who ranged from 18 to 74 years in age, were free from any systemic disease except for some kind of periodontal complaint. Of the female subjects, collection of their exudate was avoided for one week both before and after the menstruation in consideration of possible changes in the gingival state, its tone and systemic conditions. The areas of exudate collection, 3+3 and 3+3, were labially studied relative to the oral hygiene index (OHI), propounded by Greene and Vermillion, gingival index (GI) by Loe and Sillness and depths of the periodontal pockets. Upon examination, the means per tooth were classified into 3 groups. That is to say, in OHI the values below 1.5, those between 1.6 to 3.0 and those above 3.1 were designated as OHI group 1, OHI group 2 and OHI group 3 respectively. As for GI, on the other hand, the values below 1.10, those from 1.11 to 1.50 and those above 1.51 were similarly established as GI group 1, GI gronp 2 and GI group 3. Finally, concerning the mean values of pocket depth (PD), a threefold system was also adopted: those below 2.50mm was PD group 1, those between 2.51mm and 3.50mm was PD group 2 and those above 3.51mm constituted PD group 3. In collecting the exudate from the periodontal pockets, use was made of filter paper method (Whatman Co., No. 2, 1×10mm). An extraction of the phospholipids from the filter paper was carried out in accordance with the method by Folch et al. The extracted matter was evaporated to dryness under nitrogen at 45℃ and was dissolved in a 0.05ml solution of chloroform/methanol (2:1 v/v) to render it available for the purpose of Thinchrography. For the quantitative measurement of phospholipids, the Iatroscan TH-10 (Iatron Inc., TLC-FID) was employed, in which phosphatidyl-N, N-dimethylethanolamine was housed to serve the function of a internal standard. The three phospholipids to be examined were mixed with this internal standard, so that it was possible to establish the proportional relationship within a range of 1μg to 15μg for a given substance in terms of its area as compared to the internal standard matter. Subsequently, the calibration curves were prepared for the quantitative measurement and correction between each phospholipid and clinical findings were analyzed. As a result of the study, the author arrived at the following conclusions: 1. The phospholipids existing inside periodontal pockets were identified as phosphatidylcholin, phosphatidylethanolamine and sphingomyelin by use of the Thinchrography. 2. The values of phosphatidylcholin, phosphatidylethanolamine and sphingomyelin were reduced in proportion to the aggravation of the condition of oral hygiene and gingival inflammation as well. 3. As the periodontal pockets became deeper, the values of phosphatidylcholin, phosphatidylethanolamine and sphingomyelin tended to decrease.
1981-03-28

歯周ポケット滲出液中の燐脂質量の変動に関する研究 : とくにホスファチジルコリン,ホスファチジルエタノールアミン,スフィンゴミエリン量について

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