歯周疾患におけるLysozyme活性の消長に関する研究 : 〔II〕歯周疾患々者における歯肉組織内のLysozyme活性値について
スポンサーリンク
概要
- 論文の詳細を見る
Lysozyme activity of gingival tissue from patients with or without periodontal disease has been measured quantitatively in order to clarify the relationship between various periodontal conditions and the behaviour of the lysozyme activity. Following this, lysozyme activites of epithelium and tunica propria of gingival tissue were measured, and the differences of the lysozyme activity were investigated. Variations of lysozyme activity in gingival tissues were also investigated before and after oral administration of lysozyme chloride 90mg per pay for 14 days. The total subjects were 104 Japanese (adults) of the ages between 18 and 58 (including 46 males and 58 females). Gingival tissues obtained from periodontal surgery were weighed, then were immediately homogenized at 7500 rpm for 20 sec. with cold M/15 phosphate buffer solution by using a cold polytron homogenizer. The homogenate was centrifuged at 4500 rpm for 10 min. in a refrigerated centrifuge at 0℃. The supernatant fluid was used as tissue extract. Lysozyme activities of these specimens were measured by the turbidimetric method within 12 hours of storage at 4℃. The following results were obtained: 1) The total lysozyme activities in gingival tissues of the 104 individuals were very high. However, there was a considerable variation of gingival lysozyme activity in each individual. 2) The lysozyme activity of gingival tissue was found to be lowest in youth and increased with age. 3) Slight difference in the lysozyme activity of gingival tissue by sex was found, but the difference was not significant. 4) The lysozyme activity of the gingival tissue in patients with periodontal diseases was clearly higher than that of normal tissue. The activity of the tissue from acute periodontal condition was higher than that from chronic condition. A remarkable increment of the enzyme activity in proportion to the progression and aggravation of the periodontal disease was observed. Also the difference in the lysozyme activity among each periodontal group was significant. 5) The average activity of tunica propria in normal subjects was about 40.7 per cent higher than that of epithelium. The average activity of tunica propria in patients with periodontal disease was about 50.7 per cent higher than that of epithelium. 6) The lysozyme activity of epithelium and tunica propria in gingival tissues increased in proportion to the progression and aggravation of the periodontal disease. A positive statistically significant correlation was found between the activity of epithelium and tunica propria. 7) Lysozyme chloride (90mg per day for 14 days) was administered orally to patients with periodontal diseases. After the administration of lysozyme chloride, the lysozyme activity of the tunica propria was greater than that of the epithelium. 8) After oral administration of lysozyme chloride, the lysozyme activity of tunica propria in patients with severe marginal periodontitis was found to be significantly decreased. As described above, it was speculated that the lysozyme activity of gingival tissue was closely related to the various periodontal conditions. The lysozyme activity of tunica propria of gingival tissue was higher than that of epithelium. After oral administration of lysozyme chloride, the lysozyme activity of tunica propria was increased, and it is suggested that the lysozyme activity of gingival tunica propria may play an important role in the etiology and the mechanism of healing of periodontal disease.
論文 | ランダム
- 工学院大学八王子キャンパス スチューデントセンター--澤岡清秀+山本・堀アーキテクツ設計共同体[含 座談会:空間を活性化する家具]
- 大東文化大学板橋キャンパス(選定理由,作品選奨,2007年日本建築学会)
- 建築部門 審査委員会奨励賞--その他ビルの部 大東文化大学板橋キャンパス(第1期)中央棟・図書館/3号館--人と環境にやさしい都市型キャンパス (特集 「SB05Tokyo記念サステナブル建築・住宅賞」受賞作品紹介) -- (受賞作品紹介)
- 第2回 環境創造フォーラム講演会 講演 サスティナブル・キャンパス板橋の設計思想--〔大東文化大学〕板橋校舎再開発の設計に託したもの
- 工学院大学八王子校舎教室棟(工学院大学八王子キャンパス15号館)(2002年日本建築学会作品奨励賞)(推薦理由)