A new application of the SFDA-staining method to assessment of the freezing tolerance in leaves of alpine plants

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概要

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• For the first time, this study used 5- (6-) sulfofluorescein diacetate (SFDA), a fluorescent product in plant cells converted by esterase activity to fluorescein-5- (and 6-) sulfonic acid (FSA), to assess the freezing tolerance of leaf cells. We were able to readily distinguish living and dead cells, and detect differences in freezing tolerance among five alpine plants using the SFDA-staining method. We also compared this method with two conventional methods, the electrolyte leakage test and fluorescein diacetate (FDA) staining method. The electrolyte leakage test often over- or underestimated freezing injury. With the uninjured control samples, the FDA-staining method failed to stain all leaf cells, while the SFDA-staining method stained almost 100%. From these results, we concluded that SFDA-staining is a more convenient, accurate and reproducible method for analyses of freezing tolerance.

• 国立極地研究所の論文

著者

• Yamori Wataru

  Department Of Biology And Geosciences Faculty Of Science Shizuoka University

• MASUZAWA Takehiro

  Department of Biology, Faculty of Science, Shizuoka University

• Masuzawa T

  Department Of Biology And Geosciences Graduate School Of Science And Technology Shizuoka University

• Masuzawa Takehiro

  Department Of Biology Faculty Of Science Shizuoka University

• Kogami Hiroyuki

  Department of Biology and Geosciences, Faculty of Science, Shizuoka University

• Yoshimura Yoshitaka

  Faculty of Agriculture, Tamagawa University

• Tsuji Takashi

  Research Institute, Tamagawa University

• Kogami Hiroyuki

  Department Of Biology And Geosciences Faculty Of Science Shizuoka University

• Masuzawa Takehiro

  Department Of Biology And Geosciences Faculty Of Science Shizuoka University

• Tsuji Takashi

  Research Institute Tamagawa University

• Yoshimura Yoshitaka

  Faculty Of Agriculture Tamagawa University

• Tsuji Takashi

  Research Inst. Sci. & Tech., Tokyo Univ. of Sci

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