EFFECTS OF CULTURE TEMPERATURE ON MICROTUBER FORMATION OF ACONITUM CARMICHAELII DEBX. AND ACONITINE-TYPE ALKALOID CONTENTS

概要

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The clonally propagated Aconitum carmichaelii Debx. was used for the investigation of microtubering. The rooting condition of propagated shoots affected the establishment of transplantation to soil indicating the Murashige-Skoog medium supplemented with 0.5mg/1 of IAA and the cultivation at 20℃ are the best. Culturing at 15℃ under the dark enhanced microtubering rather than 10℃ or 20℃. The temperature affected the production of aconitine-type alkaloids, demonstrating that the contents of mesaconitine and hypaconitine were higher at 20℃ than at 15 and 10℃.