CHANGES IN MEDIA WATER STATUS DURING PREPARATION AND STORAGE

概要

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Significant water loss from plant tissue culture media occurs during its preparation and storage. Because medium water status has a recognized influence on culture response, any water loss and resulting increase in gelling agent and other component concentrations should be allowed for or at least accounted for during media preparation, to ensure the final media composition is that specified or expected. Two formulations of gelled-agar plant tissue culture media were prepared according to standard protocol and 42ml portions of the prepared media were dispensed into GA7 vessels for autoclaving and storage. A sample of each mixture was removed and its fresh and oven dry mass measured to determine postpreparation concentration. Likewise the postautoclave and poststorage concentrations were measured to determine the water loss and subsequent concentration increase at each step. The observed water loss indicated about a 3% increase in media agar concentration from water lost during mixing, 6% after autoclaving, and over 25% after 30 days storage in lab conditions. The loss of water under refrigerated storage resulted in considerably less change (<10%) in agar concentration over the same period.
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