キャピラリー電気クロマトグラフィー用新規機能性充填剤の開発(博士論文要録)
スポンサーリンク
概要
- 論文の詳細を見る
Capillary electrochromatography (CEC) is a hybrid microseparation technique that combines electrophoretic and chromatographic separation principle. CEC has not yet found wide acceptance as a practical tool in the chromatographic community. This narrow currency of CEC is mainly attributed to two factors: 1) much fewer examples of real sample analysis by CEC than those by HPLC; 2) a limited variation of the stationay phase specially designed for CEC. In this thesis, CEC with NAIP, 3-(1,8-naphthalimido) propyl-modified silyl silica gel, demonstrated suitability for the prompt screening of toxic drugs and the expeditious analysis of microdialysate. Good repeatability of the proposed method with a durable NAIP column was proved to be sufficient for real sample analysis. Moreover, SNAIP, 3-(4-sulfo-1,8-naphthal-imido) propyl-modified silyl silica gel, was specially designed as a CEC stationary phase and, as expected, the sulfonic acid group in SNAIP worked as a strong EOF generator. In addition to hydrophobic and π-π interactions, an electrostatic interaction participated in the retention mechanism of SNAIP. This multiple retentive mechanism indicated an advantage in the separation of peptides, which are difficult to be separated solely based on a hydrophobic interaction, and to be eluted early without high buffer concentration. The remarkable tolerance of current in CEC with SNAIP accomplished the purpose that a high concentration of buffer in mobile phase could be used for the accelerated elution of multiple charged peptides. The tolerance also enabled the use of a highly aqueous mobile phase for the separation of polar nucleosides and nucleic acid bases on a SNAIP column.
- 社団法人日本分析化学会の論文
- 2007-01-05
著者
関連論文
- 治験依頼者より提出された補償資料と同意説明文書の記載内容に関する比較検討
- 29-P3-12 悪性リンパ腫化学療法後のG-CSFの投与開始時期が白血球推移に及ぼす影響(薬物治療,社会の期待に応える医療薬学を)
- キャピラリー電気クロマトグラフィー用新規機能性充填剤の開発(博士論文要録)
- リスクマネジメント--院内での薬剤師の活動(35)長崎大学医学部・歯学部附属病院における輸液調製・処方監査に関するリスクマネジメント