Clematis huchouensis TAMURA : A Traditional Chinese Herbal Medicine and Its Quality Control Using a High Performance Liquid Chromatography Technique(Analytical Biochemistry)

概要

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A simple, specific and reliable high performance liquid chromatography (HPLC) method has been developed and validated for study of fingerprint chromatograms of extracts from the whole plant of Clematis huchouensis TAMURA (CHT) for quality control of a traditional Chinese medicinal (TCM) herb. An Agilent C_<18> column was used to separate extracts in this protocol and detection was made by ultraviolet absorbance at 340nm. The column temperature was maintained at 25℃. A mobile phase consisting of (a) water (with 30mM KH_2PO_4) and (b) CH_3CN, (c) CH_3OH was found to be suitable for this separation at a flow rate of 0.8μl min^<-1> with gradient elution. Under the described conditions, a fingerprint profile of 8 compounds was collected within 35 min, which made the HPLC method unique and interesting. The fingerprint chromatograms had good stability, precision and reproducibility. Moreover, eco-climatic (habitat) effects were studied by comparison of fingerprint chromatograms obtained from extracts of CHT collected from two habitats, with rutin as a reference marker peak. The protocol developed is quite suitable for differentiation of extracts of CHT and can be used as a quality control method for this herb and a model for other herbal drugs.
公益社団法人日本薬学会の論文
2007-01-01