マラリア感染マウス脳における胸腺外分化T細胞(IL-2R β+TCR^<int>細胞)出現の検討
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概要
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Mice were injected with 10^4 parasitized (a nonlethal strain of Plasmodium yoelii) erythrocytes and parasitemia was enumerated in the blood. Parasitemia appeared from day 5 to day 28, showing a peak at day 18. At this peak time, lymphocytes were isolated from the brain, liver, spleen and blood. The number of lymphocyte yielded by these organs increased prominently. The phenotype of lymphocytes was examined by immunofluorescence tests. Brain lymphocytes were a mixture of IL-2Rβ^-CD3^<high> cells (conventional T cells) and IL-2Rβ^+CD3^<int> cells (extrathymic T cells) before and after infection. This pattern was quite similar to that in the liver, the only difference being that NK1.1^+ CD3^<int> cells (i.e., NKT cells) which were abundant in the liver were absent in the brain. In the case of the spleen and blood, the major lymphocytes were conventional T cells, irrespective of infection. In parallel with the increase of brain lymphocytes during infection, histology of the brain indicated the accumulation of such cells in the tissue. In the blood, parasitemia and body temperature were inversely correlated, i.e., body temperature decreased with parasitemia, and monocytes increased in number in the nonlethal-strain infection. In case of the lethal-strain infection, granulocytes increased in number in the blood and these mice died within day 5. These results suggest that brain lymphocytes might be associated with resistance or severity of malarial infection and that the major such lymphocytes were extrathymic T cells.
- 新潟大学の論文
- 2006-06-10