Toxicogenomics of Kojic Acid on Gene Expression Profiling of A375 Human Malignant Melanoma Cells(Molecular and Cell Biology)

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概要

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• Kojic acid is a natural product and normally used as a food additive and preservative, a skin-whitening agent in cosmetics, a plant growth regulator and a chemical intermediate. Using DNA microarray technology, the overall biological effects of kojic acid on the gene expression profiling of a human skin A375 malignant melanoma cells were examined. After treatment with kojic acid, a total of 361 differentially expressed genes were distinctively changed with 136 up-regulated genes and 225 down-regulated genes. We used the bioinformatics tool to search the gene ontology and category classification of differentially expressed genes that provided the useful information of expressed genes belonging to cellular component, molecular function and biological process in regulation of melanogenesis. Seven down-regulated genes of APOBEC1, ARHGEF16, CD22, FGFR3, GALNT1, UNC5C and ZNF146 that were typically validated by the real-time quantitative PCR (RT-qPCR) analysis technology showed to be the tumor suppressor genes in melanoma cancer cells. Thus, microarray technology coupled with RT-qPCR offered a high throughput method to explore the number of differentially expressed genes responding to kojic acid and their biological functions, and led to more understanding of kojic acid effects on skin cancer therapy and related side effects. Moreover, the differentially expressed genes may become useful markers of skin malignant melanoma for further diagnostic and therapeutic applications.

• 公益社団法人日本薬学会の論文
• 2006-04-01

著者

• Sheu Jin-nan

  Institute Of Medicine Chung Shan Medical University

• Cheng Sun-long

  Institute Of Medicine Chung Shan Medical University

• Chen Shui-tein

  Institute Of Biological Chemistry Academia Sinica

• Chen Shui-tein

  Institute Of Biological Chemistry And Genomics Research Center:institute Of Biochemical Sciences Col

• SINCHAIKUL Supachok

  Institute of Biological Chemistry and Genomics Research Center, Academia Sinica

• HUANG LIU

  School of Nutrition, Chung Shan Medical University

• TSAY Gregory

  Institute of Immunology, Chung Shan Medical University

• Huang Liu

  School Of Nutrition Chung Shan Medical University

• Tsay Gregory

  Institute Of Immunology Chung Shan Medical University

• Sinchaikul Supachok

  Institute Of Biological Chemistry And Genomics Research Center

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• Toxicogenomics of Kojic Acid on Gene Expression Profiling of A375 Human Malignant Melanoma Cells(Molecular and Cell Biology)

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