Spectrofluorimetric Determination of Drugs Containing Active Methylene Group Using N^1-Methyl Nicotinamide Chloride as a Fluorigenic Agent

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A spectrofluorimetric method was described for the determination of drugs containing active methylene groups adjacent to carbonyl groups. The method was applied successfully to the determination of three life saving cardiovascular drugs, with narrow therapeutic indices: pentoxifylline (I), propafenone hydrochloride (II) and acebutolol hydrochloride (III), in laboratory-prepared mixtures, in commercial tablets and in plasma samples. The method involved the reaction of each of the tested drugs with N^1-methyl nicotinamide chloride (NMNCl) in the presence of alkali, followed by addition of formic acid, where highly fluorescent reaction products were produced. The produced fluorescence were measured quantitatively at 472nm (λ_<ex> 352nm), 409nm (λ_<ex> 310nm) and 451nm (λ_<ex> 266nm) for (I), (II), and (III) respectively. The method was linear over concentration ranges of 10-1000 μg/ml, 0.2-12 μg/ml and 0.08-10 μg/ml in standard solutions for (I), (II), and (III) respectively. In spiked human plasma samples, calibration graphs were linear over concentration ranges of 20-1000 μg/ml, 0.2-15 μg/ml and 0.08-10 μg/ml for (I), (II), and (III) respectively. The method showed good accuracy, specificity and precision in both laboratory-prepared mixtures and spiked human plasma samples. The proposed method is simple, with low instrumentation requirements, suitable for quality control application, bioavailability and bioequivalency studies.
公益社団法人日本薬学会の論文
2006-07-01

Spectrofluorimetric Determination of Drugs Containing Active Methylene Group Using N^1-Methyl Nicotinamide Chloride as a Fluorigenic Agent

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