A Comparative Study on Various Spectrometries with Thin Layer Chromatography for Simultaneous Analysis of Drotaverine and Nifuroxazide in Capsules

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概要

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• Three spectrophotometric methods including Vierordt's method, derivative, ratio spectra derivative, and thin layer chromatography (TLC)-UV densitometric method were developed for simultaneous determination of drotaverine HCl (DRT) and nifuroxazide (NIF) in presence of its impurity, 4-hydroxybenzohydrazide (4-HBH). In Vierordt's method, (E^<1%>_<1cm>) values were calculated at 227 and 368nm in the zero-order spectra of DRT and NIF. By derivative spectrophotometry, the zero-crossing method, drotaverine HCl was determined using the second derivative at 245nm and the third derivative at 238nm, while nifuroxazide was determined using the first derivative at 399nm and the second derivative at 411nm. The ratio spectra derivative spectrophotometry is based on the measure of the amplitude at 459nm for DRT and at 416nm for NIF in the first derivative of the ratio spectra. Calibration graphs of the three spectrophotometric methods were plotted in the range 1-10μg/ml of DRT and 2-20μg/ml of NIF. TLC-UV densitometric method was achieved on silica gel plates using ethyl acetate:methanol:ammonia 33% (10:1:0.1 v/v/v) as the mobile phase. The Rf values were 0.74, 0.50, 0.30±0.01 for DRT, NIF and 4-HBH, respectively. On the fluorescent plates, the spots were located by fluorescence quenching and the densitometrical area were measured at 308 and 287nm with linear range 0.2-4μg/spot and 0.6-12μg/spot for DRT and NIF, respectively. The proposed methods have been successfully applied to the commercial pharmaceutical formulation without any interference of excipients. Mean recoveries, relative standard deviations and the results of the proposed methods were compared with those obtained by applying the alternate methods.

• 公益社団法人日本薬学会の論文
• 2006-06-01

著者

• Youssef Nadia

  National Organization For Drug Control And Res. “nodcar"

• AYAD Magda

  Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University

• ABDELLATIF Hisham

  Analytical Chemistry Department, Faculty of Pharmacy, Zagazig University

• SOLIMAN Suzan

  National Organization for Drug Control and Research (NODCAR)

• Ayad Magda

  Analytical Chemistry Department Faculty Of Pharmacy Zagazig University

• Abdellatif Hisham

  Analytical Chemistry Department Faculty Of Pharmacy Zagazig University

• Youssef Nadia

  National Organisation For Drug Control And Research (nodcar)

関連論文

• A Comparative Study on Various Spectrometries with Thin Layer Chromatography for Simultaneous Analysis of Drotaverine and Nifuroxazide in Capsules
• Development and Validation of Spectrophotometric, TLC and HPLC Methods for the Determination of Lamotrigine in Presence of Its Impurity
• Spectrophotometric Determination of Some Drugs for Osteoporosis

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